![]() You can use any of the following programs to view your. You should see individual, sharp and evenly spaced peaksģ. Expect to get 500-700 bases of clean reliable DNA sequenceĪnything less and you might suspect contamination in your sample or consider asking your sequencing facility to apply a special protocol for a difficult template. Never trust the first 20-30 bases of a DNA sequencing read Anything more and you’re venturing into the uncertain terrain. Use a silica spin column for purification of the samples you send for DNA sequencing The peaks here are usually unresolved and small, so I suggest designing your primer at least 50bp upstream of the sequence of interest. If your sequencing facility requires you to perform your own Big Dye PCR amplification reaction (as opposed to using the all inclusive service some companies offer), you can purify the product either via the Sodium Acetate/isopropanol precipitation method or using a silica spin column available from several vendors. The precipitation method has an unfortunate side effect of messing up the reaction around base 70-75 of the read (see image below), so I would strongly recommend using a silica spin column. Open the Settings app and scroll down and tap on your name (or “iCloud” if older iOS). ![]() ![]() Tap on the email address at the very top of the iCloud settings screen.Tap on the blue text underneath the password entry that says “Forgot Apple ID or Password?” where you’ll have two choices.If you know your Apple ID and don’t remember the password, type in your email address and click “Next” to start the reset process.If you don’t know your Apple ID, tap on “Forgot your Apple ID?” and fill out your full name and email address to recover the Apple ID login (yes, you can then do the password reset after you have the Apple ID).Answer the security questions pertaining to that Apple ID, and follow the onscreen directions to complete the processĪt this point you should be good to go, you can reset the password for the account and then login as usual.What if this doesn’t work, or what if it doesn’t find your Apple ID? You can try one of the next two options: Find a Forgotten Apple ID by Email or Old Email Address This can be done with any web browser in iOS, Mac OS X, or Windows: This is a more advanced trick to search multiple email addresses, which is incredibly helpful if you switched email accounts at some point in time and that’s what has caused the login problem.
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